Service / Technology Instance

About Electron Microscopy, Brno, Czech Republic

The Cryo-electron microscopy and tomography core facility CEITEC Masaryk University (CEMCOF) is an open access facility providing access to state-of-the-art instrumentation for cryo-electron microscopy, support with cryo-EM sample preparation, and data analysis. The facility handles BSL1 and BSL2 samples, respectively, and is available to its users for both service and collaborative projects.

Instruments Available:

Talos Arctica – 200 kV high-end transmission electron microscope equipped with Ceta camera for and Falcon 3EC direct electron detector. The microscope serves for acquisition of single particle analysis data, high-throughput screening of cryo-EM samples, and acquisition of electron diffraction tomography (microED) data.

Talos F200C  - 200kV scanning transmission electron microscope for operation in both TEM and STEM modes. The microscope is equipped with Falcon 3EC direct electron detector, Ceta-D camera, and Quadro (Dectris) direct electron detection camera. In addition, the microscope is equipped with HAADF detector for imaging in STEM and Bruker X-flash detector for EDX mapping. Talos F200C is a multi-purpose microscope for life-science and chemistry applications.

Titan Krios - 300kV high-end transmission electron microscope aligned for fringe free imaging (FFI) and equipped with Volta phase plate (VPP), Falcon 3EC direct electron camera, energy filter with K2 direct electron detector (Bioquantum 967). The microscope serves for high throughput and high-quality single particle analysis and cryo-electron tomography data collection.

Versa3D – small dual beam (FIB/SEM) equipped with cryo-stage and cryo-FIB/SEM preparation system (P3010, Quorum Technologies). The microscope primarily serves for preparation of cellular lamellae by cryo-FIB for cryo-electron tomography.

User Guide

CEMCOF is a cryo-EM user facility which is a part of CEITEC, Masaryk University. The facility is located at the University Campus Bohunice in Brno, Czech Republic and is organized by the specialists who support the facility users not only in cryo-EM data collection, but also in sample preparation, screening, and data analysis. The facility continuously adopts and further develops novel approaches and workflows  in cryo-EM such as cellular lamella preparation by cryo-FIB or electron diffraction tomography (micro-ED) in order to increase the portfolio of the services provided to its users.

Provided services:

Our mission is to provide access to the state-of-the-art instrumentation for electron cryo-microscopy and support our user in sample preparation, data acquition, and data processing. Data analysis or training in different electron microscopy techniques are available through collaboration with CEMCOF. The services provided by the facility comprise:

  • high-throughput single particle analysis (SPA) data collection
  • cryo-electron tomography data acquisition
  • cryo-FIB lamella preparation
  • electron diffraction tomography (micro-ED) data acquisition
  • data analysis
  • sample preparation and cryo-EM screening
  • resin embedding
  • FIB-SEM tomography

The services are primarily provided using following key instruments:

  • Titan Krios – 300 kV high-end transmission electron microscope aligned for fringe-free imaging (FFI) and further equipped with Volta phase plate (VPP), energy filter, and Gatan K2 direct electron camera (Bioquantum 967). The microscope serves for high throughput and high-quality single particle analysis and cryo-electron tomography data collection.
  • Talos Arctica – 200 kV high-end transmission electron microscope equipped with Ceta camera for and Falcon 3EC direct electron detector. The microscope serves for acquisition of single particle analysis data, high-throughput screening of cryo-EM samples, and acquisition of electron diffraction tomography (microED) data.
  • Versa3D – small dual beam (FIB/SEM) equipped with cryo-stage and cryo-FIB/SEM preparation system (Quorum Technologies). The microscope primarily serves for preparation of cellular lamellae by cryo-FIB for cryo-electron tomography.

Instruct Centre

CEITEC

CEITEC

Kamenice 753/5

625 00

Brno

Czech Republic

www.ceitec.eu

Electron Microscopy, Brno, Czech Republic

Contacts:

Jiri Novacek
Jiri Novacek
CEITEC - Central European Institute of Technology
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Katerina Hoskova
Katerina Hoskova
CEITEC - Central European Institute of Technology
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Radka Matejickova
Radka Matejickova
CEITEC - Central European Institute of Technology
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