The Bijvoet Center at UU offers access and support for solution NMR studies of biomolecules at field strengths ranging from 500 -950 MHz with expected installation of a 1.2 GHz machine in 2021. The instruments are well-equipped for all standard structural and dynamical characterization of proteins or other biomolecules, with cryoprobes for enhanced sensitivity on three machines.
The Facility has extensive expertise in the study of protein structure, dynamics and interactions. Applications involving high molecular weight proteins or protein complexes, such as nucleosome-protein complexes (200-250 kDa) for instance, benefit from the available tailored pulse sequences, provided these samples are appropiately isotope-labelled. We can now also offer dedicated support for applications involving interactions of intrinsically disordered proteins. Finally, support for medium to high-througput screening will be offered mid 2021.
The center houses the following solution NMR machines:
In general, proteins must be isotope-labeled and prepared by the visiting scientist in advance. The Facility can offer advice on the best labeling-strategy.
Support typically includes instruments setup, data acquisition and data analysis, depending on the expertise of the visiting scientist. Support can also include training of the visiting scientist.
Access can be arranged as physical visit to the Facility or by mail-in of the samples.
Please contact the Facility staff, Andrei Gurinov and Hugo van Ingen, before submitting your proposal to align your plans optimally to our service.