Service / Technology Instance

About Proteomic Mass Spectrometry, Brno, Czech Republic

View All Mass Spectrometry at Instruct

The Proteomics core facility at CEITEC Instruct centre provides the academic and other entities with access to advanced proteomic technologies based on shared resources and the know-how of highly trained staff. Effective utilization of the state-of-the-art mass spectrometric instrumentation promotes understanding proteome complexity according to the demands of research community. Thus, the facility is involved in broad range of projects requiring protein characterisation covering fields of biochemistry, molecular and structural biology, human and veterinary medicine, microbiology, plant, agriculture and food sciences. The facility staff can offer a complete mass spectrometry-assisted proteomics services including sample preparation, separation of protein/peptide mixtures, qualitative and quantitative characterisation of proteins and their modifications by mass spectrometry and data processing.

More information can be found on the Proteomics core facility webpage.

 

Service Availability:

Remote

Instruments Available:

At present, we operate a set of mass spectrometers which allow us to cover wide range of proteomic applications:

MALDI-TOF/TOF MS:

  • mass spectrometer Ultraflextreme

LC-MS/MS systems:

  • with mass spectrometer Exploris 480 (with FAIMS)
  • with mass spectrometer Orbitrap Fusion Lumos
  • with mass spectrometer TimsTOF Pro
  • with mass spectrometers TimsTOF Pro 2

User Guide

The core facility operates in a full service mode. The services cover all of the steps of mass spectrometry-based proteomic analyses from protein isolation, protein and peptide mixture separation (liquid chromatography, gel electrophoresis), to protein characterisation by mass spectrometry and data processing. We also provide consultations and basic training in proteomics.

Long-term cooperation in the field of proteomic applications (beyond the terms of CF services) we can provide via cooperative projects with our proteomic research group.

Key Equipment

  • LC-MS/MS (with Orbitrap Fusion Lumos)
  • LC-MS/MS (with Orbitrap Exploris 480)
  • LC-MS/MS (with TimsTOF Pro)
  • LC-MS/MS (with TimsTOF Pro 2)
  • MALDI-TOF/TOF MS (Ultraflextreme)

Expertise

  • Intact mass analysis/MALDI-MS profiling
  • Protein identification
  • Characterisation of post-translational modifications
  • Protein quantification (label free, TMT)

We cover all steps of proteomic analysis - protein isolation, separation of protein mixtures, protein characterization by mass spectrometry and bioinformatics data processing.

   

Instruct Centre

CEITEC

CEITEC

Kamenice 753/5

625 00, Brno

Czech Republic

www.ceitec.eu

Proteomic Mass Spectrometry, Brno, Czech Republic

Contacts:

Zbynek Zdrahal
Zbynek Zdrahal
CEITEC - Central European Institute of Technology
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Daria Kucharova
Daria Kucharova

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Lenka Bojanovska
Lenka Bojanovska

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Klára Skopalová
Klára Skopalová

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