Service / Technology Instance

About Electron Microscopy, Heidelberg, EMBL

The cryo-electron microscopy (cryo-EM) service platform at EMBL Heidelberg gives access to state-of-the-art cryo-EM equipment for structure determination projects using the latest technology and methods in single-particle analysis and cryo-electron tomography (cryo-ET).

Instruments Available:

High-end data collection for cryo-EM single particle analysis projects. Cryo-grids will be screened with a Thermo Fisher Scientific Talos Arctica equipped with a Falcon 3 direct electron detector. High-end data acquisition will be performed with a Thermo Fisher Scientific Titan Krios equipped with a phase plate and a Gatan BioQuantum-K3 or Quantum-K2 energy filter with direct electron detector. Both microscopes are equipped with an automatic sample loading system and configured for automatic data acquisition.

High-end data collection for cryo-EM tomography projects. Cryo-grids will be screened with a Thermo Fisher Scientific Talos Arctica equipped with a Falcon 3 direct electron detector. High-end data acquisition will be performed with a Thermo Fisher Scientific Titan Krios equipped with a phase plate and a Gatan BioQuantum-K3 or Quantum-K2 energy filter with direct electron detector. Both microscopes are equipped with an automatic sample loading system and configured for automatic data acquisition.

User Guide

The EMBL Heidelberg operates the cryo-electron microscopy (cryo-EM) service platform, a user facility providing cryo-EM services that will operate before the opening of the new EMBL Imaging Centre in summer 2021. The platform is organised and managed by EM engineers and application specialists in order to help and support any scientist with cryo-EM data collection to tackle a broad range of biological questions and projects based on single-particle analysis and cryo-electron tomography for high-resolution structural determination of macromolecular complexes. The Service platform is also offering remote service to all interested users.

Currently we offer access to the following services:
1. High-end data collection for cryo-EM single-particle analysis
2. High-end data collection for cryo-electron tomography

Specific equipment:
Thermo Fisher Scientific 200 kV Talos Arctica with a Falcon 3 direct electron detector
Thermo Fisher Scientific 300 kV Titan Krios G3 equipped with a phase plate and a Gatan Quantum-K3 direct electron detector with energy filter.

The platform and its instruments are available for the entire scientific Instruct-ERIC community.

For more details on instruments, methods, experts, how to apply, and the different access modalities, please visit our website

Instruct Centre

EMBL Heidelberg

Meyerhofstra├če 1

69117, Heidelberg

EMBL

www.embl.de/services/cryo-em-platform/index.html

Electron Microscopy, Heidelberg, EMBL

Contacts:

Felix Weis
Felix Weis
EMBL Heidelberg
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Bianca Dibari
Bianca Dibari
EMBL Heidelberg
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Wim Hagen
Wim Hagen
EMBL Heidelberg
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Judith Weber
Judith Weber
EMBL Heidelberg
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Simone Mattei
Simone Mattei
EMBL Heidelberg
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